This is Curated Data
WT Data ( kinetic or thermodynamic data of wild type )
MT Data ( kinetic or thermodynamic data of mutant type )
ProteinMutation(s)_PDBProtein 1Protein 2PubMed IDWT DataMT DataMethod
4BPX_AC_BDDA111ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.65 uMfluorescence anisotropy measurements，25 °C
4BPX_AC_BDDA114ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.43 uMfluorescence anisotropy measurements，25 °C
4BPX_AC_BDDA116ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.59 uMfluorescence anisotropy measurements，25 °C
4BPX_AC_BDHA116ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.57 uM fluorescence anisotropy measurements，25 °C
4BPX_AC_BDDA306ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.48 uM fluorescence anisotropy measurements，25 °C
4BPX_AC_BDHA315ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.53 uMfluorescence anisotropy measurements，25 °C
4BPX_AC_BDKA318ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.60 uMfluorescence anisotropy measurements，25 °C
4BPX_AC_BDHA324ADNA PRIMASE SMALL SUBUNITDNA POLYMERASE ALPHA CATALYTIC SUBUNIT, DNA PRIMASE LARGE SUBUNIT24043831Kd=0.67uMKd=0.57 uM fluorescence anisotropy measurements，25 °C
4EKC_A_BRA183CGuanine nucleotide-binding protein G(q) subunit alpha Regulator of G-protein signaling 223434405Kd=20±9nMKd=22±9nMflow-cytometry-based assay，25°C
4EKC_A_BKB175AGuanine nucleotide-binding protein G(q) subunit alpha Regulator of G-protein signaling 223434405IC50=0.27 ± 0.06 uM LogEC50 (n)=2.1 ± 0.03 nM (20)IC50=0.56 ± 0.07 uM Log EC50 (n)=2.4 ± 0.03 nM (4)flow-cytometry-based assay，25°C
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